电脉冲法生产转基因鱼

The Production of Transgenic Fish by Electroporation

本文以鲤鱼受精卵为实验材料,通过电脉冲方法将全鱼基因(pcMTsGH)导入鱼胚。在处理的1873粒受精卵中(650V/cm,50μs,4次),孵出鱼苗540尾,孵化率为28.8%;经斑点杂交和Southern印迹杂交,外源基因的整合率为9.1%。因此该方法可以作为转基因鱼研究和生产的方法。

In this article the fertilized eggs of common carp (Cyprinus carpio) serve as experimental material. The all-fish gene (pcMTsGH) was introduced into common carp's fertilized eggs by electroporation. Of 1873 fertilized eggs treated with electric pulses (650 V/cm, 50 μS, 4 times), 540 (28.8%) were hatched out. The results of Dot blot and Southern blot hybridization show that the integration rate of foreign DNA is 9.1%. It indicates that electroporation technique can be used for the study and application of transgenic fish.