免疫活性细胞回输前后HCV滴度及C区和E2区CTL表位变化

Transfusion of activated immune cells induced fluctuations of HCV titer without mutations in hepatitis C virus core and E2 region CTL epitopes

目的 观察免疫活性细胞回输前后慢性丙型肝炎患者外周血丙型肝炎病毒 (HCV)滴度及C区和E2区CTL表位的变化情况。方法 定量PCR检测 4例接受免疫活性细胞回输的慢性丙型肝炎患者外周血HCV滴度 ,并利用分子克隆、基因测序和计算机辅助分析技术分析不同时间点不同分离株CTL表位的变化情况。结果 4例患者在接受免疫活性细胞回输前后血清ALT水平存在不同程度波动 ,其差值从第 2例患者的 5 8到第 1例患者的 198,同时HCV滴度也发生了较大变化 ,其变化倍数从第 3例患者的 2 5 .37倍到第 4例患者的 882 .35倍 ,其对数转换值的波动幅度在 1.4 0～ 2 .94之间。在免疫活性细胞回输后 4例患者外周血HCV滴度均出现了不同程度的一过性下降。在全部观察期内HCVC区和E2区的CTL表位编码区均未出现明确的变化。结论 机体免疫攻击状态发生改变时可引起患者体内丙型肝炎病毒滴度的变化 ,但不伴随目前观察的CTL表位编码基因的改变。

Objective To investigate the variations of HCV core and E2 region epitopes during transfusion of activated immune cells. Methods Four patients receiving transfusion of activated immune cells were under continuously observation. HCV titers were measured by quantitive PCR. HCV core and E2 regions were cloned and sequences were analyzed by computer software.Results During the follow up the serum ALT levels and the HCV virus titers fluctuated greatly in each of these persons. After transfusion, no significant variations were observed in HCV core and E2 coding regions. Conclusion The alteration of host immune attacks could induce the fluctuations of HCV load without any mutations in the currently observed coding genes of the epitopes.