高效液相色谱法测定肉中激素残留量

DETECTION OF HORMONE RESIDUE OF MEAT AND MEAT PRODUCT BY HPLC

目的 :建立 HPL C法测定肉与肉制品中己烯雌酚、雌二醇、沙丁胺醇、戊酸雌二醇、苯甲酸雌二醇残留量的方法。方法 :样品经甲醇超声萃取、离心 ,提取液浓缩后经 0 .4 5μm FH滤膜过滤 ,进高效液相色谱分析。结果 :五种激素浓度与峰面积成正比的工作曲线 ,相关系数 r>0 .9999;其变异系数 CV≤ 0 .6 (n=6 ) ,检出限≤ 3.5 ng,回收率范围为 95 .4～ 10 2 .5 %。结论 :该法具有灵敏、准确、精密、样品处理简单 。

Objective:To study a method for the determination of diethylstilbestrol,estradiol,salbutamol,estradiol valerate and estradiol benzoate residue in meat and meat product by HPLC.Methods:Estradiol,diethylstilbestrol,salbutamol,estradiol valerate and estradiol benzoate were extracted from homogenized sample with methanol,and then analyzed by HPLC.Results:Good separation was achieved in the 5 kinds of component and the standard curve showing a direct ratio between the concentration and the peak area was obtained, r>0 9999,CV≤0 6%(n=6 ).The detection limit was below 3 5ng;The recovery rate was between 95 4% and 102 5%.Conclusion:The method proves to be satisfactory in precision,accuracy and sensitivity.The preparation of sample was simple.