心脑通保护血管内皮细胞损伤的实验研究

Experimental study on protective effect of XINNAOTONG on vascular endothelial cell damage

目的 研究心脑通对血管内皮细胞的保护作用。方法 体外培养人脐静脉内皮细胞 (HUVEC) ,缺氧造成内皮细胞损伤 ,通过放免法和比色法测定培养上清液中 ET、NO水平。结果 HU VEC缺氧 4 8h后 ,NO含量明显下降 ,而 ET含量增加 ,不同浓度心脑通 (0 .2 5～ 2 .0 g/L )与 HUVEC预孵 4 8h后 ,对缺氧造成的上述变化具明显影响 ,用药后 NO含量增加 ,ET含量下降。

Object To study the protective effect of XINNAOTONG on vascular endothelial cell. Methods Human umbilical vein endothelial cells (HUVEC) were cultured in vitro and their damages were induced by hypoxia. Radioimmunoassay and colorinerric methods were used respectively to determine the effect of ET and NO levels. Results HUVEC hypoxia in 48 h, NO content decreased significantly while ET content increased, 48 h pretreatment of XINNAOTONG at different concentrations (0.25 2.0 g/L) in HUVEC affected the above mentioned changes, NO content increased while ET decreased. Conclusion XINNAOTONG has the protective effect on vascular endothelial cell damage.