三种香茶菜药材紫外光谱的除趋势对应分析

DCA on medicinal materials of three species of Isodon Kudo based on their UV spectra

目的 了解香茶菜属不同产地、同一种的不同器官在紫外光谱上的差异情况 ,明确基于紫外光谱的除趋势对应分析在药材比较上的应用前景。方法 测定了香茶菜属中的香茶菜 Isodon amethystoides、显脉香茶菜 I.ner-vosa和大萼香茶菜 I.macrocalyx不同产地和器官 15个样品的紫外光谱 ,以波数 -吸光度为指标 ,以紫外光谱为对象 ,应用除趋势对应分析 (Detrended Correspondence Analysis,简称 DCA)比较 15个样品在紫外光谱上的差异程度。结果 (1)来自不同产地的香茶菜 ,其紫外光谱图有较大差异 ;(2 )来自同一产地的同批大萼香茶菜 (或显脉香茶菜 )样品 ,它们紫外光谱图非常接近 ;(3)大萼香茶菜和显脉香茶菜与香茶菜的紫外光谱差异不很大 ;(4) 3种香茶菜的茎和叶的紫外光谱差异明显。表明不同地理源的香茶菜属植物 ,它们的药效会有较大差异 ,在香茶菜属植物入药时 ,应该注意区分茎和叶药效上的差异 ,大萼香茶菜、显脉香茶菜可以考虑作为香茶菜的代用品。结论 基于紫外光谱的

Object To observe the differences of Isodon amethystoides (Benth.) C. Y. Wu et Hsuan, I. nervosa (Hemsl) Kudo and I. macrocalyx (Dunn) Kudo from different regions in their UV spectra, to make the applicability of the method of Detrended Correspondence Analysis (DCA) clear in the comparison of Chinese medicinal materials based on the UV spectra. Methods The UV spectra of the 15 samples of I. amethystoides, I. nervosa and I. macrocalyx from different regions and organs were obtained, based on the indices of wavenumber absorbance, the differences of 15 UV spectra were compared by DCA. Results(1) the UV spectra of I. amthystoides from different regions were very different; (2) the samples of I. macrocalyx (or I. nervosa) from the same region were rather similar in their UV spectra; (3) there were resemblances among I. nervosa, I. macrocalyx and I. amethystoides in their UV spectra.; (4) the difference of UV spectra between the stems and leaves were obvious. Conclusion The above results indicate: (1) the medicinal effects of I. amethystoides from different regions may be very different; (2) the stems and leaves of I. amethystoides are of different medical effects; (3) I. macrocalyx and I. nervosa could be considered as the substitutes of I. amethystoides. It is practical to apply DCA on the basis of UV spectra to compare the chemical differences of plant samples.