溃疡病患者胃窦粘膜5-HT细胞和G细胞的变化

Changes of 5-HT and gastrin cells in human antral mucosa during peptic ulcer

目的：观察消化性溃疡病患者胃窦粘膜幽门腺5-HT细胞和G细胞的变化。方法：采用免疫组织化学PAP双重染色法，在同一张切片上显示5-HT细胞和G细胞。结果：5-HT细胞多散在于胃窦粘膜的中下1/3，呈棕褐色，形态多样，常见有突起的细胞。G细胞呈带状分布于胃窦粘膜的中1/3，大多数G细胞为蓝色，可见少数细胞为蓝黄混合色，呈圆形或锥体形。5-HT细胞数和G细胞数在胃溃疡（Gu）组分别为：4.25±1.20，7.79±2.11；十二指肠溃疡（Du）组：5.48±1.74，9.34±1.87; 对照（Con）组：3.17±1.65，6.08±1.67。5-HT细胞数和G细胞数在Gu组和Du组都增加，以Du组增加最为明显，与对照组相比均有显著性差异（P<0.01）。结论：胃窦粘膜5-HT细胞和G细胞可能参与了消化性溃疡病的内分泌调节。

Objective: To investgate changes of 5-HT and gastrin cells (G cells) in human antral mucosa during peptic ulcer. Methods: The 5-HT cells and G cells were stained with PAP double stainings on the same section. Results: The 5-HT cells were mainly distributed in mid-below third of the mucosa.The cells were brown-yellow.5-HT cells were varied in shape and most of them had long processes. The G cells were located in midzone of the mucosa. Most of them were blue and a few were bluish-yellow. The G cells appeared in different shapes such as spheroid and pyramid. The number of 5-HT cells and G cells were 4.25±1.20, 7.79±2.11 in gastric ulcer (Gu)group; 5.48±1.74, 9.34±1.87 in duodenal ulcer(Du) group ; 3.17±1.65,6.08±1.67 in control(Con) group respectively. The numbers of 5-HT cells and G cells in gastric ulcer and duodenal ulcer were higher and showed significant difference from those of the control group respectively. Conclusion: It is suggested that 5-HT cells and G cells may participate in the endocrine regulation during peptic ulcer.