欧瑞血活素对环磷酰胺模型小鼠免疫功能的影响

Effect of blood acting polypeptide injection on immunocompetence in immunosuppressed mice induced by cyclophosphamide

目的 :观察欧瑞血活素对小鼠免疫功能的影响。方法 :小鼠腹腔注射不同剂量的欧瑞血活素注射液30d ,以环磷酰胺作为模型组 ,生理氯化钠溶液为阴性对照组 ,干扰素 (腹腔注射 )为阳性对照组。结果 :与模型组比较 ,欧瑞血活素 6 0 0 ,12 0 0和 2 0 0 0mg·kg-1三个剂量组在给药最后 1d和停药后d14均显著提高中性粒细胞的杀伤能力 (P <0 .0 5 ,P <0 .0 1) ,但 2个时间点之间比较差异无显著性 ;还显著地提高小鼠溶血素水平 (P <0 .0 5 ) ;3个剂量组的碳粒廓清指数、吞噬指数、脾脏及胸腺的脏器指数均较模型组显著增加 ,其中以中剂量组增强单核巨噬细胞的吞噬功能、大剂量组增加脾脏和胸腺重量的作用最为明显。结论 :欧瑞血活素注射液可以促进小鼠的特异性体液免疫、非特异性免疫以及中性粒细胞的吞噬功能。

Objective:To observe the effect of Blood Acting Polypeptide(BAP) injection on immunocompetence in mice.Methods:The different doses of BAP were given to Kunming mice for 30 days,ip,continuously,and the model control group was treated with ip CTX,the negative control group was received ip normal saline(NS),the positive control group was received ip INF. Results:At the last day of treatment and the 14th day after treatment,the different doses of BAP(600,1 200, 2 000 mg·kg -1 ) all could increase the killing function of neutrophil in mice ( P <0.05, P <0.01 vs.model control), but there was no remarkable difference between the two points of time.The HC 50 of three doses showed significant difference compared with model control group ( P <0.05). All doses of BAP could evidently increase carbon clearance index, phagocytose index,spleen and thymus index compared with model control group.It is the most evident that the medium dose could increase the phagocytosis of mononuclear macrophages( P <0.05, P <0.01) and the high dose could profoundly raise spleen and thymus weight( P <0.01).Conclusion:BAP shows somewhat promoting effects on specific immunocompetence,non specific immunocompetence and killing function of neutrophil in mice.