忍冬花蕾化学成分研究

Studies on chemical constituents in dried buds of Lonicera japonica Thunb.

目的 :对忍冬花蕾化学成分进行研究 ,寻找抗菌活性化合物。方法 :采用溶剂分配法、硅胶柱层析进行成分提取、分离。运用UV ,IR ,MS ,1 H NMR ,1 3C NMR及标准品对照等 ,结合文献对比确定其结构 ,并进行抗菌活性药理实验。结果 :从忍冬花蕾中分离得到 11个化合物 ,分别是木犀草素 (I)、5 羟基 7,3′,4′ ,5′ 四甲氧基黄酮(Ⅱ )、5 羟基 7,3′,4′ 三甲氧基黄酮 (Ⅲ )、5 羟基 7,4′ 二甲氧基黄酮 (Ⅳ )、槲皮素 (Ⅴ )、忍冬苷 (Ⅵ )、5 ,7,3′ ,4′ 四羟基黄酮醇 3 O β D 葡萄糖苷 (Ⅶ )、3 O [α L 鼠李吡喃糖基 (1→ 2 ) α L 阿拉伯吡喃糖基 ] 2 8 O [β D 葡萄吡喃糖基 (1→ 6 ) β D 葡萄吡喃糖基 ]齐墩果酸 (Ⅷ )、绿原酸 (Ⅸ )、胡萝卜苷 (Ⅹ )、蔗糖 (Ⅺ )。 结论 :化合物Ⅳ为首次从该属植物中发现 ,化合物Ⅴ ,Ⅵ ,Ⅷ和Ⅹ为首次从该植物的花蕾中发现 ,一些化合物显示较强的抗菌活性。

Objective:To research chemical constituents from the dried buds of Lonicera japonica Thunb.and to find out the active constituents of plant.Methods:The chemical constituents of the plant were identified by means of UV,IR,MS,JH1,D,Z NMR,JC13,D,Z NMR,respectively,in some cases by direct comparison with authentic samples.Study on anti bacterial activity was carried out.Results:Eleven compounds were obtained and identified as luteolin (Ⅰ),corymbosin(Ⅱ),5 hydroxyl 7,3′,4′ trimethoxy flavone(Ⅲ),5 hydroxyl 7,4′ dimethoxy flavone(Ⅳ),quercetin(Ⅴ),lonicerin (Ⅵ), quercetin 3 O β D glucoside(Ⅶ), 3 O [ α L rhamnopyranosyl(1→2) α L arabinopyranosyl] 28 O [ β D glucopyranosyl(1→6) β D glucopyranosyl] oleanolic acid (Ⅷ),chlorogenic acid (Ⅸ),daucosterol (Ⅹ),and sucrose (Ⅺ).Conclusion:Compound Ⅳ was first isolated from Genus Lonicera ,while compounds Ⅴ, Ⅵ, Ⅷ and Ⅹ were isolated from the plant for the first time.Some compounds show anti bacterial activities.