神经生长因子对家兔单侧臂丛切断后脊髓后根神经节细胞存活数量的影响

Influence of NGF on Neurons in Dorsal Root Ganglion after an Injure of Peripheral Nerves in Rabbits

对 6例 1月龄家兔行左侧臂丛切断术 ,缝合前在神经断端给予神经生长因子 (NGF) 2 5 μg,术后 1周后 ,家兔被隔天注射NGF 1 2 .5 μg。 1个月后灌杀家兔并对其脊髓颈膨大部后根神经节的神经元数量进行计数 ,对该部位的神经细胞及核仁大小进行测量 ,以探讨NGF对家兔脊髓后根神经节在臂丛损伤后细胞和核仁的大小及细胞存活数量的影响。结果 :与单纯臂丛损伤的实验结果相比 ,从两侧比值上看更趋近等于 1。细胞计数以核仁为指标 ,且借助图像分析仪对 1例的细胞及核仁分别进行了测量。统计学分析显示 :损伤侧与未损伤侧比较 ,细胞的平均径无显著性差异 (P >0 .0 5 ) ,核仁的平均径差别亦不显著 (P >0 .0 5 )。

To investigate the effect of NGF on neurons in the dorsal root ganglion (DRG) after a injury of peripheral nerves, a unilateral transaction of the branchial plexus was done in six rabbits with the age of one month. After the transaction, 25 μg NGF was given to the stump end of the nerves before they were sutured and all animals were kept alive for one month. During this period, the rabbits were intramuscularly injected with 12.5 μg NGF every other day. The sizes of the cell bodies and nucleoli and numbers of neurons in the DRG were observed and counted under a light microscope. The ratio of the cell numbers between the injured side and uninjured side were quite close to one in the six rabbits. The nucleoli were taken as a index of calculation of cell numbers. The image analysis was used to measure the sizes of cells and nucleoli in one rabbit. The statistic analysis were used to make comparison of data from different segments and we found that there was no remarkable difference with regard to average diameter of the cells between the injured and uninjured side ( P >0.05). There was noremarkable difference on the average diameter of the nucleoli between the two sides either ( P >0.05).