辐射对树突状细胞的影响及其机理的研究

The effects of γ-radiation on dendritic cells and its mechanisms

目的 研究γ射线对树突状细胞 (DCs)生长及其功能的影响。方法 采用γ射线对单核细胞来源的DCs进行照射 ,经流式细胞仪表型分析、活细胞计数、混合淋巴细胞反应等方法测定γ射线对DCs的生物学效应。结果 γ射线照射抑制DCs的体外增殖 ,显著地下调DCs表达GM CS FR、flt 3和gp1 30分子 ,但能上调DCs表达CD80、CD86和HLA DR分子 ;同时γ射线照射增强DCs激发T细胞的增殖 ,抑制DCs对抗原摄取的能力。

Objective\ To study the effects of γ radiation on dendritic cells(DCs). Methods\ Monocyte\|derived DCs were irradiated with γ ray.Flow cytometric analysis,cell counting,and mixed lymphocyte reaction were used to study the effects of γ radiation on biological functions of DCs. Results\ γ radiation could inhibit the proliferation of DCs in vitro, down\|regulate the expression of GM\|CSFR,Flt\|3 and gp130,while up\|regulate CD80,CD86 and HLA\|DR.Moreover γ radiation enhanced DCs' potention to activate the proliferation of T cells and reduced DCs' ability for Ag capture. Conclusion\ γ radiation may effect the maturation and functions of DCs.\;