高血压脑出血患者细胞免疫及IL-2、sIL-2R表达的意义

Alteration of IL-2, sIL-2R and cell immunity in patients with hypertensive cerebral hemorrhage

目的 研究高血压脑出血患者外周血中红细胞免疫和T淋巴细胞亚群的改变以及白细胞介素 2 (IL 2 )、可溶性白细胞介素 2受体 (sIL 2R)表达的变化规律 ,探讨它们之间相互关系及临床意义。方法 采用酶联免疫吸附 (ELISA)法测定患者IL 2、sIL 2R的含量 ,免疫粘附法测定红细胞免疫活性及其调节功能 ,S P(链亲和素 过氧化物酶 )一步法测定CD3、CD4、CD8的细胞数。结果 高血压脑出血组IL 2含量较对照组明显降低 (P <0 .0 5 ) ,sIL 2R则明显升高 (P <0 .0 5 ) ;红细胞C3b受体 (RBC .C3bR)、红细胞免疫调节促进因子 (RFER)明显降低 (P <0 .0 0 1) ,红细胞免疫复合物 (RBC .ICR)、红细胞免疫调节抑制因子 (RFIR)则明显升高 ;CD3、CD4细胞数明显降低 (P <0 .0 1) ,CD8无明显差异 (P >0 .0 5 )。结论 高血压脑出血患者出现免疫功能低下。通过临床检测患者IL 2、sIL 2R含量、红细胞免疫功能、T细胞亚群活性在高血压脑出血的发生、发展、治疗。

Objective To study the presence of interleukin 2 (IL 2), soluble interleukin 2 receptor (sIL 2R) and the alteration of erythrocytic immuning and T cell subgroup in the blood of outer circulation in patients with hypertensive cerebral hemorrhage, and to explore the relationship between them and the clinical significance. Methods Enzyme linked immunosorbent assay (ELISA) was used to determine the content of IL 2 . The immunoadsorption was employed to examine the erythrocyic immune activity and its regulating function. Streptavidin peroxidase (S P) was used to determine the cell number of CD3 (cluster of differentiation 3), CD4 and CD8. Results The content of IL 2 in the group with hypertensive cerebral hemorrhage was significantly lower than that in the control group ( P < 0.01 ),and the content of sIL 2R increased. Red blood cell C3b receptor (RBC. C3bR) and RBC immune adherence enhancing factor (RFER) dropped greatly (P< 0.01 ), while red blood cell immune complex rossette (RBC. ICR) and RBC immune adherence inhibiting factor (RFIR) increased greatly. The cell number of CD3 and CD4 dropped ( P <0.01) and there was no evident change in CD8 ( P <0.05). Conclusion The decrease of immune function was observed in patients with hypertensive cerebral hemorrhage. The determination of the content of IL 2, sIL 2R, erythrocytic immunity and the activity of T subgroup has an important clinical guiding significance in the occurrence, development, treatment, outcome and prognosis of hypertensive cerebral hemorrhage.