胡萝卜与柴胡的体细胞杂交及其再生愈伤组织的同工酶鉴别

Somatic Hybridization between Carrot and Bupleurum scorzonerifoliumand Esteras Isoenzyme Analysis

目的:用体细胞杂交技术将中药柴胡的药效成分基因转入胡萝卜,为中药材的种质资源开发、优化和良种选育提供理论与实验数据。方法:取狭叶柴胡的原生质体经300μW·(cm2)-1照射O,1,2min后分别与胡萝卜原生质体用PEG(聚乙二醇)法进行体细胞杂交,对获得的再生愈伤组织进行形态学、同工酶分析。结果:初步确正9个克隆为狭叶柴胡与胡萝卜的体细胞杂种。结论:为进一步分析杂种中柴胡药效成分的含量及筛选出高药效成分含量的细胞系奠定基础。

Objective :To transfer the effective elements of Bupleurum scorzonerifolium into carrot , and provide theoretical data for the exploitation , improvement and selection of the germplasm of Chinese medicinal plants . Method : The protoplasta of Bupleurum scorzonerifolium irradiated by ultraviolet light (UV) at an intensity of 300 μW·(cm2)-1 for 0,1,2 min respectively were fused with those of carrot Fisch by PEG method . The regenerated clones, derived form a single fused cell, were examined for their hybrid nature by phenotype and Esterase isoenzyme analysis. Result:Nine clones were identified as the somatic hybrids between B. scorzonerifolium and carrot. Conclusion: This provides a firm foundation for the further analysis of the main active components saikosaponin of somatic hybrids and the screening out of high-medicine-content hybrid cell lines.