HPLC法测定马钱子及其炮制品中士的宁和马钱子碱的含量

HPLC Determination of Strychnine and Brucine in Semen Strychni and its Processed Products

目的:用HPLC法对马钱子的江西炮制方法及其改良法炮制品中士的宁和马钱子碱含量进行测定。方法:色谱柱填料为硅胶,流动相为正己烷-二氯甲烷-甲醇-浓氨溶液(47.5:47.5:5:0.35),检测波长为254 nm。结果:江西法炮制品中士的宁和马钱子碱的含量较低。结论:该法精确、简便、重现性好。

Objective:This paper reports a HPLC method for determinition of strychnine and brucine in Semen Strychni and its processed products of Jiangxi method and innovated methed. Method: SiO2 was used as the stationary phase, n-hexane-dichloromethane-methanol-ammonia(47.5:47. 5:5:0.35) as the mobile phase, with detection wavelength of 254 nm. Result: The contents of strychnine and brucine in the processed products of Jiangxi are lower. Conclusion:This method is accurate, simple and reliable.