高效液相色谱法测定马鞭草中熊果酸的含量

Derterminatiojl of Ursolic Acid in Herba of Verbena officinalis by HPLC

目的:建立马鞭草有效成分熊果酸的高效液相色谱分析方法,提高2000年版《中国药典》现有的马鞭草质量标准。方法:Kromasil C18色谱柱(4.6mm×250mm,5μm),柱温40℃,流动相甲醇-磷酸盐缓冲液(89:11),流速0.8 mL·min-1,检测波长210nm。结果:熊果酸在0.251-10.04μg呈良好线性关系,平均回收率98.1%,RSD为1.0%(n=6)。结论:本法优于2000年版《中国药典》采用的薄层扫描法,适用于马鞭草的质量控制。

Objective: To establish a HPLC method for determination of ursolic acid in dried aerial part of Verbena officinalis. Method: The column used was a Kromasil C18(4.6 mm ×250 mm) packed with a 5μm stationary phase. The mobile phase consisted of methanol-sodium phosphate buffer [monobasic sodium phosphate (MW=119.98) 1.7997 g and phosphoric acid (85%) 1.02 mL, combined and brought the total volume of 1 000 mL with water] (89:11); the mobile phase was maintained at a flow-rate of 0. 8 mL per minute; the column was maintained at 40℃; the DAD detector was set at 210 run. Results: The liner range was 0.251 -10.04μg (r=1.0000). An average recovery of 98.1 % (n=6) was obtained with a RSD of 1.0% . Conclusion: The method is simple, accurate and suitable for the qualify control.