Fcγ受体ⅢB基因型与严重牙周炎的相关关系

The relationship between Fcγ receptor ⅢB genotype and severe periodontitis in Han Nationality population

目的 :探讨Fc受体IIIB基因型与重度慢性牙周炎和广泛型侵袭性牙周炎的关系。方法 :收集四川地区汉族重度慢性牙周炎 35例 ,广泛型侵袭性牙周炎 2 5例以及牙周健康对照 95例 ,从颊粘膜拭子提取DNA ,用Fc受体ⅢB等位基因序列特异引物进行PCR扩增 ,检测基因型。结果 :3组中NA1 NA2型杂合子均占多数 ,NA1 NA1型次之 ,NA2 NA2型最少。经 χ2 检验 ,基因型分布在 3组间无显著性统计学差异。NA1、NA2基因频率为 0 .6、0 .4。结论

Objective:To study the relationship between Fcγ receptor ⅢB(Fcγ RⅢB) genotype and severe chronic periodontitis or generalized aggressive periodontitis.Methods:DNA from 35 subjects with severe chronic periodontitis,25 with generalized aggressive periodontitis and 95 of periodontal healthy controls were sampled by buccal swab and extracted using Chlex-100 method.Polymerase chain reaction with sequence specific primers (PCR-SSP)was carried out to determine the FcγRIIIB genotype.The data were analyzed with χ 2 test. Results: NA1/NA2 heterozygote was the predominant genotype in the 3 groups.No statistically differences of FcγRIIIB genotype distribution were found between any two of the stuied groups.The gene frequencies of NA1 and NA2 were 0.6 and 0.4 respectively.Conclusion:No direct relationship exists between FcγRⅢB genotype and the occurrence of severe chronic periodontitis and generalized aggressive periodontitis according to the present study.