偏侧咀嚼对生长发育期大鼠髁突软骨细胞热休克蛋白70表达的影响

Analysis of 70 kD Heat Shock Protein Expression in Condylar Cartilage of Young Rats after Unilateral Mastication

目的:初步探讨偏侧咀嚼致颞下颌关节病的致病机理。方法:间断磨除大鼠单侧上下颌磨牙至龈缘,建立生长发育期大鼠偏侧咀嚼模型,采用免疫组织化学方法检测正常对照组及实验组大鼠髁突软骨热休克蛋白70(HSP70)的表达。结果:正常组大鼠髁突软骨各带(表面带、增殖带、肥大带、钙化软骨带)均存在HSP70基础表达;实验组大鼠咀嚼侧髁突软骨细胞HSP70表达增强,而非咀嚼侧髁突软骨细胞HSP70表达无明显改变。结论:偏侧咀嚼可使生长发育期大鼠髁突软骨细胞HSP70表达增强,其致病机理仍有待进一步研究。

Objective: The purposes of this study were to analyze the expression of 70 kD heat shock protein in the condyle of young rats after unilateral mastication, and investigate the mechanism of temporoinandibular joint disease caused by unilateral mastication. Methods: A total of 12 Wistar rats aged 4 weeks were divided randomly into the control and experimental groups, and the right upper and lower posterior teeth of rats in the experimental group was ground to the gingival level without occlusal contact. HE staining and immunohistochemical methods were performed to evaluate the expression of HSP70 eight weeks after the experiment. Results: The HSP70 was expressed in different areas of the temporomandibular joints, and the staining intensity and location of the positive signals in the mastication side of the temporomandibular joint were stronger than that of the non - mastication side. Conclusion : Elevated HSP70 expression can be found in the condylar cartilage of young rats after unilateral mastication. Further study is necessary to clarify the mechanism of unilateral mastication leading to temporomandibular joint disorder.