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原发性下肢深静脉瓣膜功能不全的大隐静脉曲张致病相关基因的筛选与克隆 被引量:6

Screening and cloning of genes related to varicose great saphenous vein accompanying with primary deep vein velve insufficiency
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摘要 目的 筛选和克隆原发性下肢深静脉瓣膜功能不全的大隐静脉曲张的致病相关基因。方法 利用mRNA荧光差异显示技术比较原发性下肢深静脉瓣膜功能不全患者的曲张大隐静脉隐 股瓣膜区组织中mRNA表达与对照组的差异 ,获得的差异表达cDNA片段经Northernblot验证后进行克隆和测序以探明其基因来源。 结果 共获得 37条差异表达的cDNA条带 ,经杂交验证其中 30条为阳性 (阳性率 80 %)。克隆及测序结果显示C610 片段 (NO 18克隆 )与麦 考综合征 (Mckusick Kaufmansyndrome,MKKS)基因的mRNA序列有 96 %的同源性。 结论 C610 片段 (NO 18克隆 )与麦 考综合征基因高度同源 ,与下肢大隐静脉曲张的发病密切相关。 Objective To screen the genes related to the occurrence and development of varicosis of the great saphenous vein in the patients with primary deep vein valve insufficiency. Methods Using mRNA fluorescent differential display reverse transcriptive polymerase chain reaction (FDD-RTPCR), different genes expressed in the varicose great saphenous veins in patients with primary deep vein valve insufficiency and corresponding normal human tissues were compared. Differentially expressed cDNA fragments confirmed by Northem blot were compared and then cloned into the pGEM-Teasy vector. Positive clones were selected and sequenced. All the sequences were put into Genebank and analyzed by BLASTN software to search for their genetic origins. Results Altogether 37 different cDNA fragments were obtained and 30 of which were confirmed by Northern blot. Analysis of the sequences by BLASTN software showed that C 610 fragment (NO.18 cDNA clone) shared 96% homology with the mRNA sequence of the human Mckusick-Kaufman syndrome gene (MKKS gene). Conclusion C 610 fragment is highly homologous with the mRNA sequence of the human MKKS gene and is closely related to the development of varicosis of the great saphenous vein in patients with primary deep vein valve insufficiency.
出处 《中华外科杂志》 CAS CSCD 北大核心 2002年第12期909-911,共3页 Chinese Journal of Surgery
基金 国家自然科学基金资助项目 ( 39870 80 0 )
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  • 1王深明,胡作军,黄雪玲,李晓曦,常光其.下肢深静脉瓣膜重建术的疗效分析[J].中国实用外科杂志,2001,21(5):273-275. 被引量:35
  • 2Liang P, Pardee AB. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science, 1992, 257: 967-971.
  • 3Bauer D, Muller H, Reich J, et al. Identification of differentially expressed mRNA species by an improved technique(DDRT-PCR). Nucleic Acids Res,1993, 21: 4272-4280.
  • 4Mckusick VA, Bauer RL, Koop CE, et al. Hydrometrocolpos as a simply inherited malformation. JAMA, 1964,189:813-816.
  • 5Stone DL, Abarwala R, Schaffer AA, et al. Genetic and physical mapping of the Mckusick-Kaufman syndrome. Hum Mol Genet,1998,7:475-481.
  • 6Stone DL, Slavotinek A, Bouffard GG, et al. Mutation of a gene encoding a putative chaperonin causes Mckusick-Kaufman syndrome. Nat Genet, 2000, 25: 79-82.

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  • 1殷恒讳,张新玲,王深明,王劲松,黄雪玲.Desmuslin基因的表达下调与原发性下肢静脉功能不全关系的实验研究[J].中国病理生理杂志,2006,22(10):1926-1930. 被引量:2
  • 2王深明,朱易凡.下肢静脉疾病[M]//郑树森.外科学.北京:中国协和医科大学出版社,2005:631-643.
  • 3Beebe HG,Bergan JJ,Berggvist D,et al.Classification and grading of chronic venous disease in the lower limbs:a consensus statement[J].J Cardiovasc Surg,1997,38(5):437-441.
  • 4Hurie F,Creton D,Eklof B,et al.Prospective randomised study of endovenous radiofrequency obliteration (closure) versus ligation and vein stripping(EVOLVES):two-year follow-up[J].Eur J Vasc Endovasc Surg,2005,29(1):67-71.
  • 5Messmer K,Microcirculation in Chronic Venous Insufficiency[M].Switzer Land,Basel:Karger Publisher,1999:210-216.
  • 6Hirai M.Prevalence and risk factors of varicose veins in Japanese women[J].Angiology,1990,41(3):228-232.
  • 7Makarova NP,Lurie F,Hmelniker SM.Does surgical correction of the superficial femoral vein valve change the course of varicose disease[J].J Vasc Surg,2001,33(2):361-368.
  • 8Tang WX, Fasulo WH, Mash DC, et al. Molecular profiling of midbrain dopamine regions in cocaine overdose victims. J Neurochem, 2003,85(4):911-924
  • 9Schena M, Shalon D, Davis RW, et al. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science, 1995,270(5235):467-470
  • 10Martin KJ, Kritzman BM, Price LM,et al. Linking gene expression patterns to therapeutic groups in breast cancer. Cancer Res, 2000,60(8):2232-2238

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