抗纤合剂对血吸虫病肝纤维化的防治作用研究

Preventive and Therapeutic Effect of Kangxian Mixture on Hepatic Fibrosis Caused by Schistosomiasis in Rabbits

目的 :观察抗纤合剂的抗肝纤维化作用。方法 :用日本血吸虫尾蚴感染新西兰兔 ,制成肝纤维化模型 ,病兔均以吡喹酮顿服杀虫治疗 ,继之中药组开始服用抗纤合剂治疗肝纤维化 ,并与秋水仙碱组及 0 .9%Na Cl组比较。RIA法检测血清 型前胶原 (PC )、透明质酸 (HA) ,苏木精 -伊红、天狼红染色观察胶原沉积 ,通过原位杂交检测基质金属蛋白酶 - 1(MMP- 1)和基质金属蛋白酶组织抑制剂 - 1(TIMP- 1)的表达 ,并作 、 型胶原和转化生长因子 β1 (TGF- β1 )免疫组化染色。结果 :中药组 PC 、HA水平降低 (P <0 .0 5 ) ,图像分析显示肝组织 、 型胶原和TGF- β1 表达减弱 (P <0 .0 5 ) ,肝组织 MMP- 1m RNA的表达增强 ,TIMP- 1m RNA表达减弱 (P <0 .0 5 ) ,肝纤维化程度减轻。结论 :抗纤合剂具有抗实验性血吸虫病兔肝纤维化的作用。

Objective: To observe the anti-hepatofibrosis effect of Kangxian Mixture (KM) in rabbits infected with schistosoma japonicum. Methods: Rabbit models of liver fibrosis were established by infection of schistosoma japonicum. After treated with Praziqquantel, the rabbits were divided into three groups randomly: the first group was treated with KM, the second with colchicines, and the third with normal saline. The second and third group served as control groups. The serum levels of PCⅢ and HA were determined by RIA; Collagen deposition on liver was observed by HE staing and sirius red dyeing. The expression of TGF-β 1, typeⅠand Ⅲ collagen in liver tissue was detected by immunohistochemical technique and the expression of typeⅠand Ⅲ collagen, MMP-1, TIMP-1 mRNA in liver tissue was detected by in situ hybridization method. Results: Compared with rabbits in the control groups, the rabbits treated with KM showed the serum levels of PCⅢ, HA were decreased (P< 0.05), expression of TGF-β 1, type I collagen and type Ⅲ collagen in liver tissue was decreased (P< 0.05), and the expression of TIMP-1 mRNA down-regulated and expression of MMP-1 increased (P< 0.05). The degree of liver fibrosis in KM group was significantly alleviated as compared with that in the control group. Conclusion: KM had anti-hepatofibrosis effect on experimental hepatic fibrosis in rabbits.