早期胚胎组织中滋养细胞可溶性人类白细胞抗原G的表达

Relationship between trophoblast cell′s human leukocyte antigen G expression and early embryo development

目的 通过观察可溶性人类白细胞抗原G(sHLA G)在体外受精 胚胎移植 (IVF ET)后自然流产胚胎组织和绒毛膜癌组织中的表达 ,以探讨sHLA G在滋养细胞中的表达及其与胚胎发育的关系。方法 应用免疫组织化学标记法 ,检测 14例IVF ET(IVF ET组 )后自然流产绒毛组织和 6例绒毛膜癌 (绒毛膜癌组 )组织中的sHLA G表达情况 ,按染色深浅分为阴性、阳性及强阳性 ;并与 10例正常绒毛 (正常绒毛组 )组织进行对照。结果 (1)IVF ET组仅 1例sHLA G表达阳性 (1/14) ,其余均为阴性或弱阳性 ;绒毛膜癌组sHLA G表达全部强阳性 ;正常绒毛组sHLA G表达全部为阳性。 (2 )3组患者的sHLA G表达部位主要位于绒毛的细胞滋养细胞。结论 sHLA G在正常绒毛和绒毛膜癌的滋养细胞中呈高表达 ,在IVF ET后自然流产的绒毛组织中表达明显下降 ,表明sHLA

Objective To study the expression of soluble human leukocyte antigen G ( sHLA G) in trophoblast cells of spontaneous abortion after in vitro fertilization and embryo transfer (IVF ET) and choriocarcinoma, and explore the relationship between sHLA G expression in trophoblast cells and the early embryo development Methods 14 cases of trophoblast cells of spontaneous abortion after IVF ET and 6 cases of choriocarcinoma tissue has been detected, and which were compared with the 10 cases of trophoblast cells of normal villus Results (1)One case(1/14) has positive sHLA G expression in IVF ET group,and other cases in this group are negative or faint sHLA G expression; ALL cases in choriocarcinoma group are strong positive sHLA G expression; All cases in mormal villous group are positive sHLA G expression.(2) sHLA G is expressed on the cytotrophoblast cells, especially on the extravillus cytotrophablast The expression level of HLA G is obviously decreased in the spontaneous abortion group compared with normal group For single cells,the staining on the choriocarinoma cells are stronger than that of the normal villus cells Conclusion High expression of sHLA G in choriocarcinoma trophoblast cells and in frophoblast cells of normal villus and low expression in spontaneous abortion trophoblast cells indicating sHLA G in trophoblast cells has some relationship with the early embryo development