地塞米松对人眼小梁细胞水通道蛋白-1表达的影响

The Effect of Dexamethasone on Expression of Aquaporin-1 in Cultured Human Trabecular Meshwork Cells

目的 :观察地塞米松对人眼小梁细胞水通道蛋白 -1(AQP -1)表达的影响。方法 :通过RT -PCR方法 ,半定量检测原代培养的人眼小梁细胞AQP -1的mRNA水平及不同浓度地塞米松的作用。结果 :正常人眼小梁细胞AQP -1的mRNA(灰度比 )为1 643±0 354,10-8mol、5×10-8mol、10-7mol、5×10-7mol地塞米松作用3d为1 577±0 405、1 117±0 443、0 458±0 301、0 267±0 243。地塞米松浓度≥5×10 -8mol出现抑制作用 (P<0 05)。结论 :地塞米松使培养的正常人眼小梁细胞AQP -1的mRNA减少 ,可能是皮质类固醇性青光眼房水流出阻力增加的原因之一。

OBJECTIVE:To study the effect of dexamethasone (Dex) on expression of aquaporin-1(AQP1) in cultured human trabecular meshwork(HTM) cells METHODS:Reverse transcription combined with polymerase chain reaction(RT-PCR) was used to detect the expression of AQP1 in cultured HTM cells and those treated by Dex RESULTS:The mRNA of AQP1 expressed in normal HTM cells was 1 643±0 354,while 1 577±0 405,1 117±0 443,0 458±0 301,0 267±0 243 in those treated with Dex for 3 days in concentrations of 10-8mol,5×10-8 mol,10-7 mol,5×10-7mol As the concentrations of Dex increased to≥5×10-8mol,the expression of AQP1 was inhibited(P<0 05) CONCLUSION:The expression of aquaporin-1 is inhibited by Dex in HTM cells It may be one reason that causes the reduction of aqueous outflow in corticosteroid induced glaucoma