薄层扫描法测定升血小板胶囊中栀子苷含量

Determination of the Contents of Jasminoidin in Sheng Xuexiaoban Capsule by TLC Scanning

目的 :用薄层扫描法测定升血小板胶囊中栀子苷含量。方法 :样品经适当提取后点于硅胶GF254 薄层板上 ,以氯仿 -甲醇 (4∶1)为展开剂 ,紫外光灯 (254nm波长)下定位 ,双波长反射法锯齿扫描 ,λs=245nm ,λR=350nm。结果 :通过方法学考察 ,点样量在1μg～9μg范围内呈现良好的线性关系 ,其回归方程为 :Y=0 0903+0 1155X ,r=0 9991 ;平均回收率为98 80% ,RSD=0 45 %。结论 :该方法操作简便 。

OBJECTIVE:To establish a TLCS method for detecting the contents of Jasminoidin in Sheng Xuexiaoban capsule METHODS:After extraction,the samples were dripped on silica gel GF254 TLC plate,a mixture of chloroform:methanol(4∶1) was used as developer,locating the position under the light of an UV lamp(254nm) Double wavelength reflection sawtoothed scanning was performed λs=245nm λR=350nm RESULTS:Regression equation:y=0 0 903+0 1 155x,r=0 9 991,linear range was 1μg～9μg Average recovery was 98 80% and RSD=0 45% CONCLUSION:The method is simple,accurate and could be used to control the quality of Jasminoidin in Sheng Xuexiaoban capsule