半纤维素菌株发酵液中单糖及二糖的分析

Analysis of mono-sacchrides and di-saccharides in semi-fiber fermentation liquor by high pressure liquid chromatography

采用高效液相色谱 (HPLC)定量分析了半纤维素发酵液中的单糖及二糖 .在SpherisorbNH2 柱上用乙腈水 (体积比 82∶2 0 )为流动相分离 ,示差析光检测器检测 ,1 0min内同时分离并定量测定了六种糖 .六种糖的相对偏差均小于 2 .1 8% .

Six sugars in semifiber fermentation liquor were simultaneously determined by HPLC within 10 min.The chromatography operation was carried out on a Spherisorb NH 2 column,using acetonitrilewater(82∶18) as mobile phase and refractive index detector.RSD of the six sugars was less than 2.18%.The relation of polarity and retention time of the sugars has been analyzed.