心脉神口服液对氧化型低密度脂蛋白介导的内皮细胞ICAM-1表达变化的影响

Effect of xinmaishen oral liquid on ox-LDL-induced increase of ICAM-1 expression in human vascular endothelial cells

目的 探讨中成药制剂心脉神口服液对氧化型低密度脂蛋白 (ox LDL)致血管内皮细胞 (VEC)表面ICAM 1表达升高的保护作用。方法 以原代培养的人脐静脉内皮细胞 (HUVEC)为研究对象 ,采用细胞培养、免疫荧光染色、中药血清药理学方法、免疫荧光细胞化学染色法等技术 ,观察ox LDL对培养的VEC细胞间粘附分子 1(ICAM 1)表达的影响并研究心脉神口服液的保护作用。结果 培养液中加有ox LDL的内皮细胞ICAM 1表达明显升高 ,预先或同时加入心脉神口服液可使ICAM 1表达降低。结论 心脉神口服液能浓度依赖性地拮抗ox LDL致内皮细胞ICAM 1表达升高。

Objective To investigate the protective effect of of Xinmaishen oral liquid (XMSol) on the ICAM 1 expression increase of surface of human umbilical vascular endothelial cells (HUVECs) caused by oxidized LDL(ox LDL) . Methods Take human umbilical vascular endothelial cells(HUVEC) as study object and observe the effects of ox LDL on the cultured intercellular adhesion molecale 1(ICAM 1), and the protective effect of XMSol with the technical instrument such as cell culture, immunofluorescence, Chinese herb serum experiment, cell chemistry staining method in immunofluorescence .Results The ICAM 1 expression was obviously increased in the ox LDL group while it was decreased the control in which XMSol was put in advance ( P < 0.01 ).Conclusion The drug, XMSol, showed antagonistic effects on ox LDL enduced increase of ICAM 1 expression on HUVECs.