恶性疟原虫的克隆化及克隆株某些生物学特性的初步观察

Cloning of Plasmodium fiddpmon from Hainan Province in China and Its Characteristics

本实验用有限稀释法对海南省两株恶性疟原虫Fcc-1、Fcc-7801进行了体外克隆,共得8个疟原虫克隆。不同克隆对氯喹的敏感性相差显著,对英国抗gp195单克隆抗体、我国抗Fcc-1杂交瘤腹水的间接免疫荧光反应也有显著差异。对抗gp195单克隆抗体的反应表现具有5种类型,其中3种与McBride划分的7种血清型中的Ⅴ、Ⅵ、Ⅶ型相一致。

Two isolates of Plasmodium jalcipanm from Hainan Province were cloned by limiting dilution method and eight clones were established. Some characteristics including drug sensitivity and antigenicity of these clones were observed. The results showed that the established clones were different from each other in chloroquine sensitivity and antigenicity. The ID50 of chloroquin3e against 6 clones from isolate Fcc-7801 varied between 60.60 and 13.08 nmol/L The ID50 of chloroquine against 2 clones from isolate Fcc-1 were 93.63 and 49.64 nmol/L, respectively. According to the indirect fluorescent antibody (IFA) Teactivity of these clones with a panal of murine anti-gpl95 McAbs, 8 clones could be divided into 5-serotypes, 3 of which were consistent with the Ⅴ , Ⅵ, Ⅶ serotypes devided by J. S. McBride (1985).