不同动物棕色脂肪组织解偶联蛋白与嘌呤核苷酸结合之比较

Binding of Purine Nucleotides to Brown Adipose Tissue Uncoupling Protein from Different Kinds of Animals

以不同动物棕色脂肪组织的亚线粒体片段为标本,对解偶联蛋白与各种嘌呤核苷酸结合的亲和程度进行测定。结果表明,解偶联蛋白与嘌呤核苷酸结合的解离常数:仓鼠>豚鼠>大鼠。推测此结果是由于不同动物棕色脂肪组织解偶联蛋白多肽链氨基酸不同所致。

Using brown adipose tissue submitochondrial particles from different kinds of animals, binding of uncoupling protein to different purine nucleotides was estimated. The results indicate that the dissociate constants (Kds) of uncoupling protein from hamsters to purine nucleotides are more than the Kds of uncoupling protein from guinea pigs, which are more than the Kds of that from rats, presumably because of different amino acid residue sequences of uncoupling protein polypeptide from different kinds of animals.