脑胶质瘤浸润淋巴细胞的T亚群及抗肿瘤活性的研究

T Subsets and Antitumor Activity of Lymphocytes Infiltrating Hunan Primary Brain Gliomas

本文报告应用机械和酶消化法及不连续密度梯度离心法从9例脑胶质瘤内分离出胶质瘤浸润淋巴细胞(GIL)。在体外含白细胞介素2(IL-2)培养4周,平均扩增48.4倍,最高达118倍。新制备GIL的NK活性极低,经IL-2活化后获得选择杀伤自体胶质瘤细胞特异性。GIL中71.0+11.9％为CD_3^+淋巴细胞,其中CD_3^+多于CD细胞。新制备的GIL产生γ-干扰素显著低于IL-2活化的GIL和自体淋巴细胞。作者认为应用体外活化的GIL回输胶质瘤手术切除后的瘤腔内治疗胶质瘤具有可行性。

Glioma-infiltrating lymphocytes (GIL) were isolated from 9 surgical biopsy specimens of primary brain gliomas using mechanical and enzymatic digestion and discontinuous density gtadient centrifugation. During cultured in the presence of interieukin-2 (IL-2) for a period of four weeks, GIL were expanded 48.4-fold on the averags, even up to 118-fold. GIL activated by IL-2 had specific cytorytic activity against autologous glioma cells. Analysis of T subsets of GIL freshly isolated showed that CD3+ cells were 71.0±11.9%, CD4+ cells 34.2±6.1% and CD8+ cells 37.0±7.6%. Ability of activated GIL to secrete γ-interferon (γ-IFN) was significantly higher than that of freshly isolated GIL and autologous peripheral blood lymphocytes (PBL). The results suggest that GIL have many advantages for an adoptive immunotherapy of patients with brain gliomas and is a new type of antitumor immune effector.