胆汁酸对人红细胞膜蛋白质、酶活力和离子通透性的影响

Effects of Bile Adds on the Proteins, Enzyme Activities and Ion Permeability of Human Erythrocyte Membrane

选用人体内常见的4种胆汁酸(胆酸钠、脱氧胆酸、甘氨胆酸钠、甘氨脱氧胆酸)在生理血浆浓度(0.006mmol/L)及病理血浆浓度(0.2、0.5和1.0mmol/L)下对健康成人新鲜红细胞体外作用,观察了膜蛋白质,膜ATP酶类活力,膜乙酰胆碱酯酶活力和膜离子通透性的变化。结果表明,4种胆汁酸随其含量的增加对红细胞膜有不同程度影响。疏水性较强的脱氧胆酸和甘氨脱氧胆酸对红细胞膜酶活力及膜离子通透性的影响更明显。

Fresh healthy human erythrocytes were treated by four kinds of bile acid solutions (DC, GDC, C, and GC) at physiological and pathological concentrations of human plasma, and the membrane proteins, enzyme activities and permeability of cations were observed. The results showed that some components of the membrane cytoskeleton proteins disappeared, the activities of total ATPase and Gr-ATPase were distinctly increased with the increasing of bile acid concentrations, the activity of the membrane acetylcholinesterase was decreased with the increasing bile acid concentrations, the permeability of cations was increased with the increasing bile acid concentrations. The effect of the dihydroxy bile acids more stronger than that of the trihydroxy bile acids.