核仁组成区嗜银蛋白定量和膀胱肿瘤分级与预后的关系

Correlation between the Number of AgNOR and the Grading and Prognosis of Bladder Tumor

采用胶质银染色技术对6例膀胱正常组织、9例膀胱慢性炎症组织以及56例膀胱移行上皮癌组织的核仁组成区嗜银蛋白(AgNORs)进行了定量观察,结果表明膀胱正常组织和慢性炎症组织之间平均每核所含AgNOR颗粒数无显著性差异(P>0.05),两者平均每核AgNOR颗粒数与膀胱肿瘤相比均相差非常显著(P<0.01)。膀胱肿瘤平均每核AgNOR颗粒数随着肿瘤分级的升高而增加,且各分级之间均有明显差异(P<0.01)。此外,膀胱肿瘤平均每核AgNOR颗粒数和预后密切相关。结果提示核内AgNOR计数在区分良、恶性膀胱病变以及肿瘤分级、预后中有一定的价值。

The numbers of nucleolar organizer region (AgNOR) were counted by one-step silver colloid technique in 6 cases of normal bladder tissue, 9 chronic cystitis and 56 transitional cell carcinoma of bladder. The results revealed that the mean number of AgNOR per nucleus was not statistically different between normal bladder tissue and chronic cystitis while a significant difference was observed between normal, or inflammatory tissue and malignant tissue or between various grades of bladder tumor. The results also indicated that the mean number of AgNOR per nucleus was closely related to the prognosis of bladder tumor. So it is suggested that the AgNOR counting with silver stain technique may play a role in the differentiation of benign and malignant lesions and in the grading and prognosis of bladder tumors.