枸杞子多糖、黄芪多糖、刺五加多糖和鼠伤寒杆菌内毒素多糖对LAK活性的调节作用

Influences of Lyciun Barbarian Polysaccharide, Astragalus Polysaccharide, Polysaccharide of Acanthopanax Senticosus and Polysaccharide of Lipopolysaccharide on the Induction of LAK Cells in vitro

采用^(125)IUdR释放法测定了不同浓度的枸杞子多糖、黄芪多糖、刺五加多糖及鼠伤寒杆菌内毒素多糖对不同剂量rIL-2激活的杀伤细胞(LAK)抗肿瘤活性的影响,发现4种多糖在一定浓度条件下均可在体外增强LAK活性。枸杞子多糖在0.01～0.1mg·ml^(-1)、黄芪多糖和刺五加多糖在0.01mg·ml^(-1)时增强作用最显著(P<0.001),内毒素多糖在0.0001～0.1μg·ml^(-1)时可增强LAK活性,在0.0001μg·ml^(-1)时最显著。4种多糖浓度过低或过高则不能增强,甚至抑制LAK活性。

Effects of Lycium barbarian polysaccharide (LBP), Astragalus polysaccharide (APS), polysaccharide of Acanthopanax senticosus (PAS) and polysaccharide of lipopolysaccharide (PS) on cytotoxicities of LAK cells from splenocytes of C57BL/6 mices were observed with 18 h 125IUdR release assay. Our study demonstrated that the polysaccharides alone were shown to induce no cytotoxicities. When combined with rIL-2, all of 4 kinds of the polysaccharides augrnented LAK activities in dose-dependent manner. The augmentations reached the maximum at 0.01-0.1 mg · ml-1 LBP, 0.01 mg · ml-1 APS and PAS, 0.01 mg · ml-1 PS. They were found to increase LAK activities in short range of rIL-2 concentrations (250-1000U· ml-1). If the polysaccharides were used beyond the suitable concentrations, they were shown to inhibit LAK cell activities.