摘要
作者对抗原制备进行政进,先磺化5′-CMP,将其与蛋白质载体相连,再用此抗原免疫BALB/c小鼠,经细胞融合,筛选和多次克隆化,选择出一株高特异性和敏感性的单抗(B_(10))。B_(10)可以在dot blot中检出0.05pg的磺化DNA,而10ng未磺化DNA不显色,其特异性与国外同类产品相似,敏感性则较高。本研究为国内研究DNA,RNA提供了良好的工具。
In this study, 5'-CMP was sulfonated, and then the modified 5'-CMP was connected to a protein carrier as an immunogen to immunize BALB/c mice. After cell hybridization, screening and recloning , a McAb (B10) with high sensitivity and specificity was selected. In a dot Hot using the McAb B10, less than 0.05 pg of sulfonated DNA could be detected while 10 ng of DNA was not coloured The result showed that the sensitivity of McAb B10 was higher than that of the McAb from 'Chemiprobe' kit
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1992年第4期343-346,共4页
Academic Journal of Second Military Medical University
关键词
DNA探针
小鼠
单克隆抗体
antibody, monoclonal
DNA probes
animal
mice
