视黄酸对人骨肉瘤细胞增殖分化的影响

Effects of Retinoic Acid on the Proliferation and Differentiation of Human Osteosarcoma Cells

本文报道了视黄酸(Retinoic acid,RA)对人骨肉瘤细胞系HOS-8603细胞增殖分化的影响。研究结果表明,不同浓度(10^(-6)～10^(-10)mol/L)的RA均可不同程度地抑制HOS-8603细胞的增殖,而且具有明显的剂量依赖性特点:RA(10^(-8)mol/L)还可明显地抑制HOS-8603细胞的克隆增殖。碱性磷酸酶活性测定表明,RA可使HOS-8603细胞碱性磷酸酶活性提高,结合RA可使HOS-8603细胞形态发生改变,提示RA对HOS-8603细胞的分化过程也有一定影响。不同浓度的RA与10^(-8)mol/L地塞米松联合作用时,低浓度时呈现协同作用,但高浓度时则表现为拮抗作用。

The effects of retinoic acid (RA) on the proliferation and differentiation of a newly established human osteosarcoma cell line, HOS-8603, were studied. The results showed that RA could inhibit the proliferation of HOS-8603 cells at different concentrations ranging from 10-6 to 10-10 mol/L, and the inhibitory effect of RA was in a dose-dependent manner. In addition, RA (10-8 mol/L) could also significantly suppress the clonal proliferation of HOS-8603 cells. The effects of RA on the alkaline phosphatase activity in HOS-8603 cells were also determined. It was found that the alkaline phosphatase activity could be induced 48 h after treatment with RA (10-7 mol/L). The induction of alkaline phosphatase activity and morphological changes in HOS-8603 cells by RA suggested that RA might play roles in the differentiating processes. The interactions between retinoic acid and dexmathasone (Dex), a synthetic glucocorticoid, in the control of HOS-8603 cell proliferation were also investigated, and it was found that RA at low concentrations (10-8 to 10-10 mol/L) had a synergistic effect, while high concentrations of RA (10-6 and 10-7 mol/L) had a antagonistic effect with Dex on the proliferation of HOS-8603 cells.