烫伤大鼠比目鱼肌氧化还原状态变化与蛋白质分解代谢的关系

Relationship between the state of oxidation-reduction and protein degradation in the scalded soleus muscle of rats

本文以一侧后肢(7％体表面积)全层烫伤大鼠为模型观察伤后比目鱼肌蛋白质分解速率与氧化还原状态的关系。结果指出伤后72h大鼠烫伤肢离体保温及在体比目鱼肌细胞内乳酸/丙酮酸比值、苹果酸/丙酮酸比值明显低于未伤肢,蛋白质分解速率升高。胰岛素(0.01u/ml介质)能显著升高比目鱼肌细胞内乳酸/丙酮酸与苹果酸/丙酮酸比值,同时降低蛋白质分解速率。从而提示:烫伤后大鼠比目鱼肌蛋白质分解速率的变化与胞液中氧化还原状态密切相关。

One hind leg(7 %TBSA)of the rat was scalded and the changes of the reduction-oxidation state and protein degradation in the soleus muscle were observed 72 hours postinjury both in vitro and in vivo.It was found that the lactate/pyruvate (L/P) and malate/pyruvate(M/P)ratios of the soleus muscle were significantly lower and the protein degradation rate much higher in the scalded leg than in the unscalded legs and the control.After the addition of insulin to the medium significant elevation of L/P and M/P ratios and reduction of the protein degradation rate in the soleus muscle could be observed.There findings suggest that there is a good correlation between the changes of the reduction-oxidation state and the protein degradation rate in the cytosol of the soleus muscle after scalding in the rat.