肠系膜上动脉夹闭性休克大鼠肝线粒体损伤及抗损伤的实验研究

Studies on hepatic mitochondrial injury and their anti-injury capacity after superior mesenteric artery occlusion shock in the rat

本文在大鼠肠系膜上动脉夹闭(SMAO)休克后,观察了肝线粒体呼吸功能的变化,并比较了多种自由基清除酶(剂)对肝线粒体功能的保护作用。松夹1 h,实验组RCR较对照值明显下降(P<0.05),而P/O值无明显减少。松夹2 h,RCR下降更甚(P<0.01),P/O值亦下降明显(P<0.05)。表明SMAO休克时,肝线粒体呼吸功能严重障碍,ATP合成减少。松夹1 h后,ALLO组、SOD+CAT组,SOD+ALLO组和SOD+ALLO+CAT组的RCR,P/O值均较对照值无明显下降(P>0.05),而较实验组明显升高(P<0.01),松夹2 h后,ALLO组RCR虽较对照值有明显降低(P<0.01),但仍明显高于实验组(P<0.01)。其它各联合治疗组RCR,P/O值均仍较对照值无明显减少。各治疗组动物平均存活时间均显著高于实验组。表明各自由基清除酶(剂)对SMAO休克时,大鼠肝线粒体功能有不同程度的保护作用,以联合治疗效果较好。

Changes of hepatic mitochondrial respiratory function and the protective effects of several free radical scavenging enzymes or drugs on the mitochondrial functions were observed after superior mesenteric artery occlusion shock was inflicted to the rat.It was found that there was an obvious decrease of respiratory control rate (RCR) in the 1st hour after the occlusion was released and a further decrease in the 2nd hour,and P/O value decreased significantly at the same time,which indicates that mitochondrial dysfunction does occur.in the shock due to superior mesenteric artery occlusion.However,RCR ard P/O value did rot significantly decrease in the 1 st hour after occlusion releasing in the treated groups with allopurinol (ALLO),a combination of catalase (CAT) and superoxide dismutase (SOD),a combination of SOD and ALLO,and a combination of SOD,ALLO,and CAT as compared with those of the control,and were markedly higher as compared with those of the experimental group.In the 2nd hour after occlusion releasing,RCR of the ALLO treated group was significantly lower than that of the control and remained significantly higher than that of the experimental group.The average survival time of the animals was much longer in the treated groups than in the experimental group.Our findings demonstrate that free radical scavengers,SOD,CAT,and ALLO especially a combinaton of them can exert protective effects of different degrees on the mitochondrial respiratory function during a shock due to superior mesenteric artery occlusion.