活性氧在犬实验性呼吸窘迫综合征发病中的作用

Study on the role of active oxygen in the pathogenesis of respiratory distress syndrome in dogs

将成龄杂种犬25只,随机分3组:致伤组、治疗组、盐水组。采集不同时相动、静脉血及活杀时的肺组织,肺泡灌洗液(BAL),测超氧化物歧化酶(SOD)活性与脂质过氧化物(LPO)含量,结合临床、病理学检查,得以下结果:(1)复制了骨髓型呼吸窘迫征(RDS)模型;(2)致伤组伤后血液、肺组织、BAL中SOD和LPO明显升高,提示注射骨髓提取液后,活性氧(AO)的产生急剧增加,过氧化作用增强,出现不同程度的急性肺损伤表现;(3)治疗组伤后血、肺组织和BAL中SOD和LPO虽亦升高,但均低于致伤组,提示由于654-2的干预,AO产生减少。盐水组未见异常。结果提示,AO在犬RDS急性肺损伤中可能发挥了重要作用。

Twenty-five mongrel dogs were randomized into 3 groups;the animals of group Ⅰ were traumatized with bone marrow extract,those of group Ⅱ were similarily traumatized and then treated with anisodamine,and those of group Ⅲ received saline only and served as control.The specimens of arterial and venous blood were collected and bronchoalveolar lavage fluid (BALF) and lung homogenate were obtained after the animal was killed.The activity of superoxide dismutase (SOD) and the concentration of lipid peroxides (LPO) were determined.Meanwhile,the clinical manifestations,blood gas analysis.chest radiography,and pathological examinations were performed or observed.It was found there were following findings:(1)In group I,SOD activity and LPO level in the lung homogenate and BALF were markedly elevated immediately after injury,which suggests that there had been a rapid production of active oxygen.As a result.various degrees of lung damages were pricipitated.(2)In group Ⅱ,an increase of SOD activity and LPO level in the blood,lung homogenate and BALF:The elevation was more marked than that in group Ⅲ but less marked than that in group Ⅰ.which indicates that there was a relatively of less amount of active oxygen production by the interference anisodamine.(3)In group Ⅱ,no significant changes of SOD activity and LPO livel were found.Our findings suggest that active oxygen is likely to play a very important role in the pathogenesis of acute lung damages in respiratory distress syndrome.