复方抗瘤冲剂抑制小鼠S180肉瘤生长的机理研究

Mechanism of Compound Antitumor Granules in Inhibition of S180 Growth on Mice

目的探讨复方抗瘤冲剂对S180肉瘤的作用及机制。方法通过体内给药,测定抑瘤率及应用流式细胞仪测定S180肉瘤细胞的凋亡率、细胞内Bax及细胞黏附分子CD54的表达量。结果不同剂量复方抗瘤冲剂均有抑瘤作用,抑瘤率分别为19.76％、26.85％和29.94％,抑瘤率与剂量有正相关性,并且对肿瘤细胞有诱导凋亡的作用。Bax的表达水平用药后有明显升高,细胞周期用药前后没有显著改变。复方抗瘤冲剂对CD54表达也有明显的抑制作用。结论复方抗瘤冲剂的抑瘤活性与其诱导细胞凋亡有关,实验结果表明凋亡是通过上调Bax的表达水平来实现的。

Objective To investigate the anti-tumor effect of Compound antitumor granules on mice bearing sarcoma 180 and its possible mechanism. Methods The weight of tumor treated with different dosages of Compound antitumor granules was weighed. The apoptosis rate and the expression of Bax and CD54 were measured by flow cytometry. Results Compound antitumor granules can inhibit S180 growth at various extent, the rates of inhibition were 19.76% , 26. 85% and 29.94% respectively, and it can significantly induce sarcoma 180 cell apoptosis and inhibit the expression of CD54 ,and the inhibition of S180 growth, the apoptosis rate and the inhibition of CD54 are dependent on the dosage of compound antitumor granules. Compared with the control, the cell cycle changed slightly. Conclusions The antitumor activity of compound antitumor granules is related to the induction of sarcoma180 cell apoptosis, and the sarcoma180 cell apoptosis is attributed to compound antitumor granules upregula-tion of expression of Bax.