大鼠肝、脾、肺对疟原虫子孢子的拘捕及与巨噬细胞活性的关系

Relationship of capture of circulating P.yoelii sprozoites by liver,spleen and lungs with the activity of macrophages in rats

大鼠感染^(125)I标记的约氏疟原虫子孢子后2h,子孢子在肝、脾、肺的含量分别为1.67％、0.046％、0.49％。2～18 h子孢子在肝的分布下降缓慢;脾内基本保持恒定;肺内下降较快。活体灌注子孢子,较大流速不影响肝对子孢子的拘捕率,但使肺的拘捕率显著降低。巨噬细胞吞噬活性增加的大鼠肝、脾脏内子孢子含量高于巨噬细胞吞噬活性受抑制的大鼠,肺内子孢子含量不受巨噬细胞吞噬活性影响。实验结果表明肺对子孢子的拘捕主要为机械性拘捕,肺巨噬细胞在子孢子入侵过程中的作用不大;肝、脾对子孢子的拘捕以巨噬细胞的生物性拘捕为主。

Two hours after the rats were infected with 125I - labeled sporozoites of Plasmo-dium yoelii,the sporzoites captured by the liver,the spleen and the lungs accounted for 1.67%,0.046% and 0.49% respectively.During the 2nd to the 18h,the sporozoites decreased in number slowly in the liver,remained unchanged in the spleen and decreased in number quickly in the lungs as time went on.When the liver and the lungs were perfused with quick M199 stream containing sporozoites,the capture rate of sporozoites was not affected in the liver but that of the lungs was markedly decreased.The numbr of sporozoites in the liver and the spleen was larger in those rats with their phagocytosis activated than in those with the phagocytosis inhibited.while in the lungs,the number of sporozoites was not influenced by the activity of macrophages.These findings suggest that the mechanism of capturing sporozoites in the lung is mainly mechanical and the alveolar macrophages play little role in that,but that in the liver and the spleen is mainly a biological process in which macrophages play an important role.