斑点酶联免疫吸附试验检测伤寒特异性抗体

Dot-ELISA in determination of specific antibody of typhoid

目的 验证斑点酶联免疫吸附试验 (Dot -ELISA)检测伤寒抗体的敏感性和特异性。方法 采用Dot -ELISA测定伤寒沙门菌包膜抗原V、菌体抗原O和鞭毛抗原Hd 的特异性抗体 ,并与肥达氏反应相比较。结果 肥达氏反应均为阴性 ,而经Dot -ELISA试验 ,疑似伤寒患者血清中检出 3种抗体阳性 1例 ,非伤寒发热病人血清中检出单项Hd 抗体阳性1例。Dot -ELISA法比肥达氏反应敏感 10倍以上 ,且无交叉反应。结论 该法有较高的特异性和敏感性 ,且操作简单、快速、无需特殊设备 ,宜于临床推广应用。

Objective To evaluate the sensitivity and specificity of Dot-ELISA in determination antibody of typhoid. Methods A Dot-ELISA method was used to determine the specific antibodies of envelope antigen, O antigen and H d antigen in Salmonella. Then the results were compared with the results of Widal's reaction. Results The antibodies tested by Widal's reaction were all negative. But in Dot-ELISA test, 3 kinds of antibodies in the serum of one of the cases doubted with typhoid were positive, and single H d antibody was also positive in the serum of one of the cases with non-typhoid fever. Dot-ELISA was ten times more sensitive than Widal's reaction, moreover, it had no cross-reaction. Conclusions Dot-ELISA has higher specificity and sensitivity, the operation is easy and simple, and it is unnecessary for special equipment. So it could be recommended to widely use in clinical.