泌尿道革兰氏阴性杆菌抗生素耐药性及其质粒图谱的分析

Analysis of antibiotk resistance and plasmid profile of Gram-negative bacilli isolated form a urologic unit

本文对由泌尿科住院患者尿液中分离的28株革兰氏阴性杆菌进行了抗生素耐药谱及其质粒图谱的分析。结果表明:有92％以上的分离株对氨苄青霉素、庆大霉素、氯霉素、荼定酸、环丙氟哌酸和洁霉素耐药;且所有分离株均至少对5种以上的抗生素耐药。有13个分离株(46.4％)带质粒,其中以分子量为50Md的质粒出现率最高,该质粒可能为泌尿科内的流行质粒。本文还发现来自医院内两泌尿道感染者的分离株虽为不同菌种,却显示相同的质粒图谱,提示此两感染患者可能存在某种相互联系。

Twenty -eight strains of Gram-negative bacilli isolated from the urine of the patients ot a urologic unit were studied for their antibiotic resistance and their plasmid profile.It was found that 92% of the strains were resistant to most of antibiotics used in the unit except cephalothin.amikacin and polymyxin B>and each of the resistant strains was resistant at least to 5 antibiotics.13 strains out of the 28 harbored plasmids ranging from 1 to 6 in number and 105 Md to 2.7 Md in molecular weight.The plasmid with 50 Md was the most popular one in this study,which may be the epidemic plasmid in the urologic unit.It was also found that 2 different species of bacteria isloated from the patients with infection of the urinary tract showed identical plasmid profile,which indicates that these 2 patients may be associated with each other.