重度烧伤狗肝、肾线粒体呼吸功能及ATP含量的变化

Changes of mitochondrial function and hepatic and renal ATP level after severe burn injury in dogs

本文用呼吸速率、呼吸控制率(RCR)、磷氧比值(ADP/O)和ATP含量为指标,观察了50％TBSA Ⅲ°烧伤狗肝、肾线粒体呼吸功能的变化。 结果发现,烧伤后肝、肾线粒体呼吸速率增强,而RCR、ADP/O和ATP含量有明显降低,肝分别降至正常对照组的43.1％、70.6％和40.1％;肾分别降至73.4％、76.2％和51.4％,差异非常显著(P<0.001)。伤后立即输液组肝RCR、ADP/O和ATP分别是正常对照组的96.5％、95.4％和89.8％;肾分别是88.7％、93.5％和85.0％,均较接近正常对照组,差异不显著(P>0.05)。结果提示,重度烧伤严重损害肝、肾线粒体正常生理功能,可致线粒体氧化磷酸化生成ATP能力降低。而伤后立即输液对肝、肾线粒体功能有明显保护作用。

Respiratory control rate(RCR),ADT/O ratio and ATP content of the liver and kidneys were determined to evaluate the effects of 50% TBSA third degree burns on the respiratory function of the mitochondria in the liver and kidneys in dogs.It was found that the stage 4 respiratory rate was increased significantly postburn(P<0.01).RCR.ADT/O ratio and ATP level were significantly decreased to 43.1%,70.6% and 40.1% in the liver and 73.4%,76.2% and 51.4% in the kidneys as compared with those of the normal control(P>0.01).Yet in the group of dogs with immediate fluid replecement after burn injury,RCR,ADT/O ratio and ATP level were 96.5%,95.4% and 98.8% in the liver and 88.7%,93.5% and 85.0% in the kidneys as compared with those of the normal control(P>0.05).These findings indicate that severe burns can destroy the coupled phosphorylation in the mitochondria and impair their capability to produce ATP.Immediate fluid infusion after burns can protect the mitochondrial function effectively.