胃癌组织中癌基因激活和抑癌基因失活的研究

A study on the activation of oncogene and inactivation of suppressor gene in human gastric cancer

本文采用Southern杂交技术分析了42例胃癌及癌旁组织中癌基因c-Ha-ras、K-ras、N-ras、c-myc、N-myc、hst、EGFR、c-erbB-2和抑癌基因p53和Rb的变化;使用PCR-RFLP方法分析了p53基因第248和249位密码子的点突变。结果显示,胃癌组织中有多种癌基因激活和抑癌基因失活,但多集中在c-Ha-ras、c-erbB-2、hst、EGFR和p53基因。这些结果表明,多种癌基因和抑癌基因可能参与了胃癌的发生和发展。

The changes of several oncogenes and suppressor genes in the specimens of cancerous and juxtacancerous tissues of 42 cases of gastric cancer were studied with Southern blot hybridization and PCR-RELP method.The probes used were c-Ha-ras,K-ras,N-ras,N-myc,c-myc,hst,EGFR,c-erbB-2,p53 and Rb.Amplification,rearrangement,and deletion of c-Ha-ras were detected in 8/33 (25.8%) cases of gastric cancer (amplification or rearrangement of hst and c-erbB-2 in 11/42 (26.6%) and 12/42 (29.2%) cases respectively (amplification of EGFR in 21.4% of cas-esideletion or rearrangement of p53 and Rb in S/30 (30%) and 2/15 (13%) cases respectively) and amplification or rearrangement of N-ras (0/33),K-ras (1/26),N-myc (1/26),and c-myc (1/ 35) was only rarely encounted.The point mutation in codon 248 and 249 of p53 in gastric cancer was analyzed.2 cases of the 42 harbored point mutation in codon 248 of p53.These findings suggest that e-Ha-ras,hst,c-erbB-2,EGFR and p53 may be the hot point genes in the occurrence and development of gastric cancer.