三氧化二砷诱导舌鳞状细胞癌Tca8113细胞凋亡的实验研究

Preliminary Study on Arsenic Trioxide Induced Tca8113 Cell Apoptosis

目的观察三氧化二砷对舌癌细胞系Tca8113的诱导凋亡作用。方法以人舌鳞癌细胞系Tca8113为研究对象,使用荧光染色光镜、透射电镜观察三氧化二砷不同浓度、不同时间作用后的肿瘤细胞的形态学变化,流式细胞仪检测细胞凋亡率。结果一定浓度的三氧化二砷作用Tca8113细胞24h、48h后,细胞形态学观察可见有明显的细胞凋亡发生;流式细胞仪检测Tca8113细胞的基础凋亡率为0.5%～1.2%,三氧化二砷作用后肿瘤细胞的凋亡率可提高到15%～20%。结论三氧化二砷可诱导人舌鳞癌细胞系细胞凋亡。

Objective To explore the possibility of arsenic trioxide inducing the apoptosis in the treatment of oral squamous cell carcinoma. Methods Human tongue squamous cell carcinoma cell line Tca8113 was used for in vitro studies. The effect of arsenic trioxide on oral squamous cell carcinoma was studied by using flow cytometry, fluoroscope and electric microscope. Results Arsenic trioxide induced Tca8113 cell apoptosis, while inhibiting the growth and survival. Conclusion Arsenic trioxide might be one drug of inducing apoptosis in the treatment of oral squamous cell carcinoma.