摘要
AIM To study the anticancer effects of two novel highly oxygenated bisabolance sesquiterpene compounds from Cremanthodium discoideum Maxim on the human hepatoma cell line SMMC-7721. METHODS In vitro, the cytotoxity of compound 1, 2 was tested by MTT colorimetric assay, and the single-cell gel electrophoresis (SCGE) assay was used for assessing the DNA damage of SMMC-7721 cells induced by compound 1, 2. The apoptotic rate of SMMC-7721 cells was assayed by flow cytometry. RESULTS The IC 50 of SMMC-7721 cells exposed to the two compounds for 72 h were (37±7) μg·mL -1 and (22±9) μg·mL -1, respectively. The result of SCGE showed that the two compounds (2, 4 and 8 μg·mL -1, 48 h) were able to induce DNA damage in SMMC-7721 cells and showed dose-dependent effect. Flow cytometric analyses showed that after exposure to the two compounds for 48 h induced the apoptosis in SMMC-7721 cells. CONCLUSION The two novel highly oxygenated bisabolance sesquiterpene compounds showed significant inhibition effect on the growth of SMMC-7721 cells and its mechanism was probably related with the DNA damage and apoptosis induced by them.
AIM To study the anticancer effects of two novel highly oxygenated bisabolance sesquiterpene compounds from Cremanthodium discoideum Maxim on the human hepatoma cell line SMMC-7721. METHODS In vitro, the cytotoxity of compound 1, 2 was tested by MTT colorimetric assay, and the single-cell gel electrophoresis (SCGE) assay was used for assessing the DNA damage of SMMC-7721 cells induced by compound 1, 2. The apoptotic rate of SMMC-7721 cells was assayed by flow cytometry. RESULTS The IC 50 of SMMC-7721 cells exposed to the two compounds for 72 h were (37±7) μg·mL -1 and (22±9) μg·mL -1, respectively. The result of SCGE showed that the two compounds (2, 4 and 8 μg·mL -1, 48 h) were able to induce DNA damage in SMMC-7721 cells and showed dose-dependent effect. Flow cytometric analyses showed that after exposure to the two compounds for 48 h induced the apoptosis in SMMC-7721 cells. CONCLUSION The two novel highly oxygenated bisabolance sesquiterpene compounds showed significant inhibition effect on the growth of SMMC-7721 cells and its mechanism was probably related with the DNA damage and apoptosis induced by them.
出处
《药学学报》
CAS
CSCD
北大核心
2002年第12期993-995,共3页
Acta Pharmaceutica Sinica
基金
甘肃省科技攻关项目 (GS0 0 3 A5 2 0 2 2 )
