不同治疗对哮喘模型大鼠气道顺应性和肺病理变化的影响

Influence of Different Treatments on Airway Compliance and Lung Pathology in Asthma Rats

为探讨中医不同治疗对哮喘的疗效与机理,将SD大鼠用卵蛋白、灭活百日咳杆菌、氢氧化铝干粉混合液腹腔注射以致敏后,用2％卵蛋白雾化吸入诱发哮喘发作。然后分别以宣肺固本方(主药:生地黄、熟地黄、党参、泽泻、黄精、淫羊藿、南沙参、北沙参、半夏、地龙、黄岑)、宣肺方(主药:麻黄、半夏、地龙、柴胡、黄芩、葶苈子)、补肾方(主药:生地黄、熟地黄、党参、茯苓、黄精、玉竹、淫羊藿)水煎剂灌胃,共3周,并与西药顺尔宁、地塞米松比较。观察各组大鼠治疗后的气道顺应性(Co,Cp),肺与气管的FN、CF,及肺与气管的病理切片。结果:三种不同治疗对哮喘模型均有一定疗效,均能降低Cp、FN、CF,并可减轻肺泡壁中嗜酸性粒细胞浸润和肺部病变,其中尤以宣肺固本组为佳。提示宣肺固本法是治疗哮喘的有效方法。

To investigate the clinical efficacy of different treatments in treating asthma and its mechanism, the SD rats were sensitized by intra-abdominal injection of mixed ovalbumin, inactivated bacillus pertussis and aluminum hydroxide,and then induced to have asthma by inhaling 2% ovalbumin.They were randomized into five groups and respectively treated by 'Xuefei Guben Decoction', 'Xuanfei Decoction','Bushen Decoction',singulair and dexamethasone for 3 weeks. After treatment, the airway compliance(Cp), FN and CF as well as pathological sections in lungs and trachea were observed. Results: The former three treatments could reduce Cp, fibronectin (FN), collagen fibers (CF) and the infiltration of eosinophil in alveolar wall and lungs, the first treatment in particular. It is revealed that diffusing lungs and nourishing kidneys is an effective method for asthma.