硫代巴比妥酸不除蛋白质快速测定过氧化脂质

Rapid determination of lipid peroxides by thiobarbituric acid reaction without precipitating protein

作者用硫代巴比妥酸(TBA)呈色,经对试剂进行调整,不使蛋白质沉淀直接测定血清(浆)过氧化脂质(LPO).本法准确可靠,简便快速,灵敏度较高,稳定性及精密度好.批内CV=3.62％、批间CV=4.45％,平均回收率为99.68％,线性范围良好(1～40 nmol/ml),呈色后2 h十分稳定,与荧光法比较呈显著的正相关(r=0.785,b=0.756).50例正常人血清LPO含量(x±S)9.58±2.15 nmol/ml,血浆(肝素)为7.31±1.27 nmol/ml.并对部分干扰物作了初步探讨,血红蛋白达50mg％,呋喃(口旦)啶8mg％,磺胺嘧啶8 mg％、胆红素10mg％时对测定结果有明显干扰.

A method for determination of lipid peroxides (LPO) was established with thiobarbituric acid reaction without precipitating protein. This method was characterized by good accuracy, high precision, stability as well as rapidity, In group CV of this method was 3.62% and the between group CV was 445%. It also had a good linary in 1 - 40 nmol/ml, with average recovery as 99.68%. The correlation between this method and fluorescenceassay method was significant (r= 0.785, b=0.756), the color was stable within 2 h . The normal LPO ranges of 50 healthy individuals were 9.58±2.15 (x ±S, sera) and 7.31 ±1.27 (x ± 5, plasma) differently. Many substances interfered with the detection were observed haemoglobin with 50 mg%, furationum with 8 mg%, suliadiazinum and bilirubin with 8 mg% and 10 mg%.