HX-1号液保存大鼠胰腺24及48小时的胰腺移植研究

A study on pancreas preservation with HX-1 solution for 24 hours and 48 hours in rat pancreas transplantation

本实验将大鼠胰腺分别用HX - 1号液和CollinsⅡ液保存 2 4小时和 4 8小时 ,然后移植给药物性糖尿病大鼠 ,通过检查受鼠的血糖、糖耐量试验及胰腺病理表现。探讨HX - 1号液保存大鼠胰腺的效果。结果发现HX - 1号液和CollinsⅡ液保存大鼠胰腺 2 4小时后移植的两组受鼠的血糖 ,糖耐量试验结果无统计学差异 (p >0 0 5 ) ,与正常大鼠对照组也无统计学差异。在保存 4 8小时的两组中 ,HX - 1号液的保存效果明显优于CollinsⅡ液 ;其血糖值较保存 2 4小时的两组稍高 ,但无统计学差异 ,而糖耐量试验结果受到损害。CollinsⅡ液保存 4 8小时后移植 ,经上述各项指标检测 。

The study was performed to examine hypotherimic preservation of pancreas with HX-1 solution in rats.The pancreases were preserved by both HX-1 solution and CollinsⅡ solution for 24 and 48 hours.Then isografts were performed on the streptozotocin induced diabetic rats.Blood glucose,glucose tolerance tests of the recipients were tested after transplantation.Pathology of grafts were examined 15 days after transplantation.The results of the blood glucose,glucose tolerance tests of recipients presented no significant difference in 24 hour preserved pancreas between by HX-1 and by CollinsⅡ solution(P>0.05).There was no significant difference compared with normal control group.The pancreases were better preserved in HX-1 solutiion than CollinsⅡ solution for 48 hours,but worse than 24 hour preserved pancreas in both solution,the Blood glulose of recipients which received 48 hour preserved pancreas in HX-1 solution were slightly higher than that of recipient with 24 hour preserved pancreas in both solutions,but the difference was not significant.However,the glucose tolerance tests were abnormal.When the pancreas was preserved by CollinsⅡ solution for 48 hour,the graft almost lost it's function after transplantation.