艾灸大椎穴对慢性应激大鼠神经营养因子的影响

The effect of neurotrophic factor in chronic irritability rats with moxibustion at dazhui point

目的 :观察艾灸大椎穴对慢性应激状态下大鼠海马神经元及脑源性神经营养因子的影响。方法 :SD雄性大鼠 18只 ,按随机表分为正常组、模型组、艾灸组。应用孤养和长期不可预见性的中等强度刺激造成应激失调模型 ,观察各组大鼠海马神经元的变化。采用HE染色、尼氏体染色的方法光镜下观察海马神经元形态的改变 ,用免疫组织化学的方法对海马脑源性神经营养因子 (BDNF)阳性神经元进行染色 ,并用图像分析仪定量分析。结果 :慢性应激可致大鼠海马神经元明显受损 ,神经营养因子阳性神经元数量亦显著减少 ,形态以空泡为主。艾灸大椎穴对此有明显改善作用。结论 :艾灸大椎穴对慢性应激大鼠脑源性神经营养因子有良性调节作用 ,并对海马神经元有保护作用。

To observe BDNF of moxibustion on stress.Method:18 male Sprague-Dawley rats were randomly divided into control group,model group and Moxibustion group.All rats except the control group singly housed and exposed chronically 21 days to a variety of mild unpredictable stressors,and the rats of Moxibustion group accepted Moxibustion treatment at the same time.Use immunocytochemistry method and computer to quantitatively analyse and With method of histochemistry and immuno-histochemistry,we observe the structure of hippocampal neurons and measure the BDNF infected neurons.Results:Compared with control group,the number of hipoccampal neurons decreased with cell structure destroyed.Immuno-histochemistry results showed that the number of infected nerons of model animals was much less than that of normal animals.Moxibustion'da zhui'can improve behavioral disorder.And that moxibustion can protect the hippocampal neurons and increase the number of BDNF neurons in hippocampus in the depressed animals.Conclusion:Moxibustion'da zhui'can increase the number of BDNF neuron in hippocampus and protect the hippocampus neurons.