摘要
为探讨迷走神经刺激 ( Vagus nerve stimulation,VNS)抗癫痫的作用机制 ,应用原位杂交组织化学及图像分析方法研究戊四氮 ( Pentylenetetrazol,PTZ)致痫大鼠丘脑网状核谷氨酸受体 NMDAR1m RNA和 γ-氨基丁酸 A受体( GABAAR)α1亚单位 m RNA的变化。结果显示 ,PTZ致痫组大鼠丘脑网状核 NMDAR1m RNA表达明显高于正常对照组 ,而 VNS抗癫痫组明显低于 PTZ致癫痫组。与之相反 ,PTZ致癫痫组 GABAARα1m RNA的表达明显低于正常对照组 ,VNS抗癫痫组明显高于 PTZ致癫痫组。上述结果表明 ,VNS可能通过抑制丘脑网状核兴奋性神经递质受体 NM-DAR的活动和增强抑制性神经递质受体 γ-氨基丁酸受体 GABAAR的活动 ,降低大脑皮层的兴奋性 。
To study the antiepileptic mechanism of vagus nerve stimulation (VNS), we used the methods of in situ hybridyzation and image analysis to detect the expression of NMDAR1 mRNA and GABA A receptor alpha1 subunit mRNA (GABA ARα1 mRNA) in the thalamic reticular nuclus. The results show that the NMDAR1 mRNA expression of rats administered pentylenetetrazole(PTZ) is higher than that of control group. By treating with VNS, it decreased. On the contrary, the expression of GABA ARα1 mRNA in the thalamic reticular nuclus of PTZ group rats is lower than that of control group. For rats treated with VNS, it increased. Therefore, it is concluded that VNS may reduce the excitability of cerebral cortices by depressing the activities of glutamic acid receptors(GluR) and by promoting the activities of gamma-aminobutyric acid receptors(GABAR) in thalamic reticular nuclus. So the formation and development of seizures are inhibited.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
2002年第4期566-568,F002,共4页
Journal of Biomedical Engineering
基金
湖北省自然科学基金资助项目 ( 99J12 6)
