摘要
从新生大鼠真皮组织中分离多能干细胞 ,体外长期传代培养 ,观察细胞形态和超微结构的变化 ,检测细胞增殖和分化能力的改变及胶原基质对细胞生长的影响 ,揭示体外长期传代培养对大鼠真皮间充质干细胞生物学性状的影响。结果表明 :大鼠真皮组织中的间充质干细胞体外培养至 180 d,传至 2 5代的细胞仍具有干细胞的特征 :形态均一 ,细胞核结构原始 ,细胞器不发达 ;体外增殖迅速 ,保持了向成骨细胞和软骨细胞分化的潜能 ;胶原基质成分可促进细胞的生长 ,而胶原海绵支架更利于细胞的三维生长。结论是体外长期传代培养后大鼠真皮间充质干细胞仍保持良好的干细胞特性 。
Autologous multipotent stem cells are most relevant cells for regenerative medicine and show prosperous future in the treatment of human diseases.Previous reports have indicated that multipotent stem cells (MSCs) can be obtained from bone marrow and adipose tissues.In this study,we proved that dermis may be another source of these cells. MSCs were isolated from the dermis of newborn rats one day old by adhesion competition and successive culture.These cells conserved the ability to differentiate to osteoblasts,chondrocytes and adipocytes by induction media containing dexamethasone.After long term of more than 6 months,till 25th generation, the cells still maintained the characteristics of stem cells:high activity of self-renewal and multipotency.Mixed collagen matrix from dermis could promote the growth of dermis-derived multipotent stem cells and collagen sponge stent could promote their three-dimensional growth in vitro.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
2002年第4期660-663,共4页
Journal of Biomedical Engineering
基金
国家重点基础研究发展规划资助项目 ( G19990 5 42 0 5 )
