对-呋喃硝苯啶对离体兔心缺血再灌注损伤的保护作用

Protective effects of para-fural nifidipine on injury of ischemia and reperfusion in isolated rabbit hearts

对-呋喃硝苯啶为钙拮抗剂硝苯啶的呋喃衍生物.为观察该药的心肌保护作用,作者采用离体兔心缺血再灌注损伤模型,以灌流液中CPK,LDH,GOT以及血浆中自由基代谢中间产物MDA含量、红细胞中SOD活力为指标,选择缺血前、缺血10min,再灌5,30,60 min5个时间点取样,发现6.07×10^(-6)和1.2×10^(-5)mol/L的对-呋喃硝苯啶呈剂量依赖性地减少心肌3种酶的释放,并使再灌60 min时MDA含量由空白的2.58±0.40降至1.76±0.15 mmol/ml(P<0.01),SOD活力由1184.23±180.18提高到1496.6±112.76U/ml(P<0.01).说明该药对心肌缺血再灌注损伤有一定的保护作用,此作用与减少心肌组织自由基的产生有关.

Para-fural nifidipine is a fural derivant of Ca-A nifidipine synthesized recently by Northwest University PRC. Experiment of its protective effects on myocardium, a recirculating nonpulsatile perfusion circuit was done and it was found that p-Nif 6.07 × 10-6 mol/L and 1.2 ×10-5 mol/L could decrease the leakage of CPK, LDH, GOT of myocardium in the prosesses of ischemia and reperfusion. It could also decrease MDA from 2.58±0.40 in preischemia to 1.76±0.15 nmol/ml when reperfused for 60 min, and raise the activity of SOD from 1 184.23±180.18 to 1496.6±112.71 U/ml (F<0.01). All the changes were related with drug concentration. This results indicated that the mechanism of p-Nif protecting the reperfusion myocardium might associate with inhibiting the production of free radicals.