rIL-2对慢性HBV感染者淋巴细胞转化功能的影响

The effect of rIL-2 on lymphocyte transformation function of patient with chronic HBV infection

本文采用~3H-TdR渗入技术,观察到慢性HBV感染者休外淋巴细胞转化功能受到明显的损害。HBsAgrHBeAg和rHBeAg可明显抑制正常人淋巴细胞转化,且有剂量依赖性。上述情况在加入外源性重组白细胞介素Ⅱ(rIL-2)后仍不能得到纠正。实验提示,慢性HBV感染者淋巴细胞转化功能低下的原因除了IL-2生成减少外,还有HBV或其产物对它的直接抑制或通过某种机制间接干扰。

The lymphocyte transformation function of patient with chronic HBV infection was measured in the presence or absence of exogenous rIL-2 , HBsAg, rHBeAg and rHBeAg. The response of the patient to PHA was significantly impaired as compaired with that of the control. The impairment could not be repaired by rIL-2over a wide range of concentrations. HBsAg, rHBeAg and rHBeAg showed a significant inhibition effect on lymphocyte transformation. This effect was dose-dependent and exogenous rIL-2 could not influence it. Thus, the reduction of lymphocyte transformation of patients with chronic HBV infection not only should be attributed to deficiency of IL-2 production, but also to direct inhibition or indirect interference with the PHA-induced lymphocyte transformation via a certain mechanism by HBV or its products.